Nanoparticles Platform

NanoGetic SL provides a full range of services related ti customize nanoparticles (NPs). The company was founded to exploit the know-how of Founders in the flield of nanotechnology. The have developed a variety of protocols to prepare NPs based oncross-linked polystyrene including magnetic and fluorescently labeled products. These NPs by bio-orthogonal strategies following multi-step solid phase organic synthesis.

– Preparation of multifuncionalized cross linked polystyrene NPs.

– Mono-dispersed polymeric nanoparticles with selec:

      • Particle size (100nm-10 micrometers)
      • Size distribution 
      • Surface modification 
      • Capacidad de carga (high, medium or low)

–  Egineered  nanoparticles (ENPs):

      • Our nanoparticles are designed to be flexible to be linked with different molecular tags.
     

– Services of Scale Up Production of nanoparticles. NanoGetic expertise is focussed in scaling-up the production of nanoparticles. From small scale lab production to a reliable, safe and cost-effective standard protocol for big scale production following GMP.

– Services of Quality Control of nanoparticles:

  – In Vitro Characterization:

  • Physico-chemical characterization: Size, Size distribution, Zeta Potential, pH of nanoparticle suspensions, Drug loading, Drug release in simple and complex media.
  • Stability in biological media
  • Microbial contamination
  • Endotoxin
  • Detection of Mycoplasma
  • Cytotoxicity
  • Immunotoxicity
  • Hemocompatibility

 – In Vivo Characterization: Toxicity and pharmacokinetic studies. In vivo imaging. Customized service. Design of a set of assays depending on the application of     the nanomaterial.

– Aplications:
     •  Targeted drug delivery
     • Personalized medicine
     • Diagnostic 
     • Basic research 
     • Smart material 

Camelid single domain antibody platform

NanoGetic team is composed by scientists with recognized experience in the generation of camelid single-domain antibody (sdAb) phage display libraries. Camelids produce a fraction of a unique G-like immunoglobulin isotype that is naturally devoid of the Light chains, named Heavy-chain antibodies (HcAb). The variable domains (VHH) of these HcAbs can be obtained, through recombinant gene technology, as sdAbs. These recombinant sdAbs are the smallest available intact antigen-binding fragment (12-15 kDa) harboring the full antigen recognition capacity. Their exceptional physicochemical properties, possibility of humanization and unique antigen recognition properties make them excellent candidates for targeted delivery of biologically active components.

– Immune libraries. NanoGetic S.L. construct these libraries by cloning the VHH genes repertoire, produced by peripheral blood lymphocytes of immunized dromedary with the antigen of interest, in a property phage-display plasmid to finally select antigen-specific VHH fragments (sdAbs).

– Non-immune (naïve) libraries. NanoGetic own a larger dromedary sdAbs phage display library to select naturally occur specific sdAbs.

– Humanization. sdAbs can be humanized by changing the amino acids 49 (E–G) and 50 (R/C–L) in the framework-2 maintaining their properties and specificity.

– sdAbs coupling to nanodevices.

Custom peptides and peptidomimetics synthesis platform

NanoGetic SL use standard Fmoc-based solid-phase chemistry to synthesize customized peptides and peptidomimetics such as peptoids. We have the experience and knowledge to offer custom peptides solutions to our customer. For this we use highly optimized protocols for peptide synthesis.
The peptides are supplied in different degrees of purity depending on the customer´s requirement (crude products or>70 %, >85%, >90% or 95% purity).
Peptides include mass spectrometry and HPLC chromatography to confirm purity and identity.
Contact our technical support (technical@nanogetic.es) and tell us what do you need and we will give you our advice about the best synthetic approach for your peptide. You will receive a very competitive price quotation.